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Table 2 Identification of S. aureus with the common tests in comparison to PCR-detection of the nuc gene

From: Identification of Staphylococcus aureus: DNase and Mannitol salt agar improve the efficiency of the tube coagulase test

Other tests

nuc PCR

 

Outcome

Positive (% of 32)

Negative (% of 28)

Subtotal

MSA

Positive

30 (94)

6 (21)

36

 

Negative

2 (6)

22 (79)

24

DNase

Positive

24 (75)

1 (4)

25

 

Negative

8 (25)

27 (96)

35

1Human plasma

Positive

29 (91)

25 (89)

54

 

Negative

3 (9)

3 (11)

6

Human plasma/MSA

Positive

27 (84)

6 (21)

31

 

Negative

0

3 (11)

3

Human plasma/DNase

Positive

21 (60)

1 (4)

22

 

Negative

0

3 (11)

3

Human plasma/MSA/DNase

Positive

21 (60)

1 (4)

22

 

Negative

0

3 (11)

3

2Sheep plasma

Positive

26 (81)

26 (93)

52

 

Negative

6 (19)

2 (8)

8

Sheep plasma/MSA

Positive

24 (75)

6 (21)

30

 

Negative

0

2 (8)

2

Sheep plasma/DNase

Positive

18 (56)

1 (4)

19

 

Negative

0

1 (4)

1

Sheep plasma/MSA/DNase

Positive

18 (56)

1 (4)

19

 

Negative

0

2 (8)

2

MSA/DNase

Positive

24 (74)

1 (4)

25

 

Negative

2 (6)

22 (79)

24

  1. 1: tube coagulase (human plasma)
  2. 2: tube coagulase (sheep plasma)
  3. MSA: Mannitol salt agar
  4. DNase: Deoxyribonuclease
  5. PCR: Polymerase Chain Reaction