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Table 2 Identification of S. aureus with the common tests in comparison to PCR-detection of the nuc gene

From: Identification of Staphylococcus aureus: DNase and Mannitol salt agar improve the efficiency of the tube coagulase test

Other tests nuc PCR
  Outcome Positive (% of 32) Negative (% of 28) Subtotal
MSA Positive 30 (94) 6 (21) 36
  Negative 2 (6) 22 (79) 24
DNase Positive 24 (75) 1 (4) 25
  Negative 8 (25) 27 (96) 35
1Human plasma Positive 29 (91) 25 (89) 54
  Negative 3 (9) 3 (11) 6
Human plasma/MSA Positive 27 (84) 6 (21) 31
  Negative 0 3 (11) 3
Human plasma/DNase Positive 21 (60) 1 (4) 22
  Negative 0 3 (11) 3
Human plasma/MSA/DNase Positive 21 (60) 1 (4) 22
  Negative 0 3 (11) 3
2Sheep plasma Positive 26 (81) 26 (93) 52
  Negative 6 (19) 2 (8) 8
Sheep plasma/MSA Positive 24 (75) 6 (21) 30
  Negative 0 2 (8) 2
Sheep plasma/DNase Positive 18 (56) 1 (4) 19
  Negative 0 1 (4) 1
Sheep plasma/MSA/DNase Positive 18 (56) 1 (4) 19
  Negative 0 2 (8) 2
MSA/DNase Positive 24 (74) 1 (4) 25
  Negative 2 (6) 22 (79) 24
  1. 1: tube coagulase (human plasma)
  2. 2: tube coagulase (sheep plasma)
  3. MSA: Mannitol salt agar
  4. DNase: Deoxyribonuclease
  5. PCR: Polymerase Chain Reaction
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Annals of Clinical Microbiology and Antimicrobials

ISSN: 1476-0711

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