Table 1 Scorpion primers and primer sequences
Oligonucleotide | Mutations | Sequence |
|---|---|---|
23SF2 | - | 5'-TGCGAAATTCCTTGTCGG-3' |
23SSc | WT | 5'-FAM AAGGTAGGTGAAAATTCCTCC TACC BHQ1 HEG GGACCACGGGGTCTTT-3' |
23Ssc | A2143G | 5'-Texas red AAGGTAGGTGAAAATTCCTCC TACC BHQ2 HEG GGACCACGGGGTCTTT-3' |
23Ssc | A2142G | 5'-Cy3 AAGGTAGGTGAAAATTCCTCC TACC BHQ1 HEG GGACCACGGGGTCTTC-3' |
23Ssc | A2142C | 5'-Cy5 AAGGTAGGTGAAAATTCCTCC TACC BHQ1 HEG GGACCACGGGGTCTTG-3' |