Rapid detection of Pseudomonas aeruginosa from positive blood cultures by quantitative PCR

Table 2 ecfX qPCR testing of P. aeruginosa from 98 positive blood culture bottles

Organisms	No. of samples detected		Total no. of samples
	PCR ⁺	PCR ^-
*Pseudomonas aeruginosa*	33	0	33
Escherichia coli	0	21	21
Stenotrophomonas maltophilia	0	9	9
Klebsiella pneumoniae	0	8	8
Enterobacter cloacae	0	7	7
Citrobacter koseri	0	4	4
Enterobacter aerogenes	0	3	3
Proteus mirabilis	0	3	3
Morganella morganii	0	3	3
Serratia marcescens	0	1	1
Pantoea spp.	0	1	1
Brevundimonas diminuta	0	1	1
Bacteroides fragilis	0	1	1
E. coli + Klebsiella oxytoca^a	0	1	1
E. cloacae + K. oxytoca^a	0	1	1
K. pneumoniae + *P. aeruginosa*^{a, b}	0	1	1

^a Polymicrobial blood cultures. ^b, 20 CFU/ml of P. aeruginosa and 10⁸ CFU/ml of K. pneumoniae were recovered by quantitative culture.

ISSN: 1476-0711