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Table 1 Oligonucleotide primers and LightCycler hybrizidation probes used in the PCR assay

From: Rapid detection of Pseudomonas aeruginosa from positive blood cultures by quantitative PCR

Oligonucleotides

Sequence (5'-3')

Target gene

Nucleotide position

ecfX-F

TTCCATGGCGAGTTGCT

ecfX b

46-62

ecfX-R

CGGGCGATCTGGAAAAGAA

 

179-197

ecfX-HP-1

GCTGAAATGGCCGGGCC-[FAM]

 

135-151

ecfX-HP-2

[LC705]-CAATCGGTCGAGCAGCCGC-Ph

 

154-172

  1. a [FAM], fluorescein; [LC705], LightCycler™-Red 705; Ph, 3'-phosphate. b Extracytoplasmic function gene (GenBank accession no. DQ996551).
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Annals of Clinical Microbiology and Antimicrobials

ISSN: 1476-0711

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