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Table 1 Oligonucleotide primers and LightCycler hybrizidation probes used in the PCR assay

From: Rapid detection of Pseudomonas aeruginosa from positive blood cultures by quantitative PCR

Oligonucleotides Sequence (5'-3') Target gene Nucleotide position
ecfX-F TTCCATGGCGAGTTGCT ecfX b 46-62
ecfX-R CGGGCGATCTGGAAAAGAA   179-197
ecfX-HP-1 GCTGAAATGGCCGGGCC-[FAM]   135-151
ecfX-HP-2 [LC705]-CAATCGGTCGAGCAGCCGC-Ph   154-172
  1. a [FAM], fluorescein; [LC705], LightCycler™-Red 705; Ph, 3'-phosphate. b Extracytoplasmic function gene (GenBank accession no. DQ996551).