Table 1 MIC (μg/ml) of the antimicrobial agents, ceftazidime (CAZ), cefotaxime (CTX), cefpodoxime (CPD), ciprofloxacin (CIP), gentamicin (GEN) and kanamicin (KAN), and PCR amplification results of the resistance-encoding genes on selected conjugative isolates and their corresponding E. coli J53 (sodium azide-R) transconjugants, in comparison to their RAPD profiles.
Isolate number (parents and transconjugants) | MIC of antimicrobial agents | Resistance-encoding genes | RAPD profile | |||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Â | CAZ | CPD | CTX | CIP | GEN | KAN | aac (6')lb-cr | ISEcp1- bla CTX-M-15 | qnrS. | Â |
E. coli J53 | 0.19 | 0.064 | 0.75 | 0.047 | 0.5 | 1.5 | - | - | - | Â |
E. coli 1 | >256 | >256 | >256 | >32 | 96 | 12 | + | + | + | E-d |
E. coli 1 T | 32 | >256 | >256 | 3 | 16 | 6 | + | + | + | Â |
E. coli 3 | 16 | >256 | >256 | 0.125 | 32 | 12 | + | + | - | E-j |
E. coli 3 T | 16 | >256 | >256 | 0.125 | 16 | 8 | + | + | - | Â |
E. coli 6 | 24 | >256 | >256 | >32 | 1.5 | 16 | + | + | - | E-l |
E. coli 6 T | 16 | >256 | >256 | 0.125 | 0.75 | 6 | + | + | - | Â |
E. coli 8 | 16 | >256 | >256 | >32 | 128 | 32 | + | + | - | E-r |
E. coli 8 T | 16 | >256 | >256 | 0.094 | 12 | 8 | + | + | - | Â |
E. coli 11 | 24 | >256 | >256 | >32 | >256 | 64 | + | + | - | E-l |
E. coli 11 T | 16 | >256 | >256 | 0.125 | 16 | 16 | + | + | - | Â |
E. coli 12 | 24 | >256 | >256 | >32 | 64 | 24 | + | + | - | E-l |
E. coli 12 T | 12 | >256 | >256 | 0.125 | 16 | 4 | + | + | - | Â |
E. coli 14 | 16 | >256 | >256 | 0.094 | 16 | 3 | + | + | - | E-b |
E. coli 14 T | 8 | >256 | >256 | 0.047 | 1.5 | 6 | + | + | - | Â |
E. coli 15 | 24 | >256 | >256 | >32 | 1.5 | 24 | + | + | - | E-q |
E. coli 15 T | 24 | >256 | >256 | 0.125 | 1.5 | 4 | + | + | - | Â |
K. pneumoniae 1 | >256 | >256 | >256 | >32 | 128 | 48 | + | + | - | K-e |
K. pneumoniae 1 T | 32 | >256 | >256 | 0.125 | 16 | 8 | + | + | - | Â |
K. pneumoniae 2 | 32 | >256 | >256 | 0.25 | >256 | 32 | + | + | - | K-o |
K. pneumoniae 2 T | 16 | >256 | >256 | 0.125 | 12 | 8 | + | + | - | Â |
K. pneumoniae 13 | 96 | >256 | >256 | 0.5 | 1.5 | 4 | + | + | - | K-g |
K. pneumoniae 13 T | 24 | >256 | >256 | 0.047 | 0.5 | 4 | + | + | - | Â |
S. sonnei 1 | 12 | >256 | >256 | 0.047 | 2 | 8 | - | + | - | S-a |
S. sonnei 1 T | 12 | >256 | >256 | 0.047 | 0.5 | 1.5 | - | + | - | Â |
S. sonnei 3 | 12 | >256 | >256 | 0.047 | 2 | 8 | - | + | - | S-a |
S. sonnei 3 T | 12 | >256 | >256 | 0.047 | 0.5 | 1.5 | - | + | - | Â |
S. sonnei 4 | 12 | >256 | >256 | 0.047 | 2 | 8 | - | + | - | S-a |
S. sonnei 4 T | 12 | >256 | 96 | 0.047 | 0.5 | 1.5 | - | + | - | Â |