A diagnostic PCR assay for the detection of an Australian epidemic strain of Pseudomonas aeruginosa

Table 4 Sensitivity of TaqMan PCR for detection of oprL and the AES-I HW2 locus

DNA per reaction (ng)	Genome equivalents/reation^a	*oprL*		HW2
		Average Ct TAMRA ^b,d	Standard Deviation	Average Ct FAM ^c,d	Standard Deviation
1000	2.2 × 10⁸	25.44	1.99	16.30	0.47
100	2.2 × 10⁷	29.13	3.19	19.57	0.27
10	2.2 × 10⁶	32.77	2.86	23.00	0.68
1	2.2 × 10⁵	35.18	2.23	26.40	0.95
0.1	2.2 × 10⁴	37.47	1.04	30.19	0.90
0.01	2.2 × 10³	-	-	33.23	0.84
0.001	2.2 × 10²	-	-	36.70	1.46

^a based on weight of P. aeruginosa PAO1 genome of 4.5 fg
^b oprL correlation coefficient r² = 0.9865, amplification efficiency = 114.84%
^c HW2 correlation coefficient r² = 0.9996, amplification efficiency = 96.84%
^d Fluorescent dye used for primer labeling; see Materials and Methods.

ISSN: 1476-0711