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Table 4 Sensitivity of TaqMan PCR for detection of oprL and the AES-I HW2 locus

From: A diagnostic PCR assay for the detection of an Australian epidemic strain of Pseudomonas aeruginosa

DNA per reaction (ng) Genome equivalents/reationa oprL HW2
   Average Ct TAMRA b,d Standard Deviation Average Ct FAM c,d Standard Deviation
1000 2.2 × 108 25.44 1.99 16.30 0.47
100 2.2 × 107 29.13 3.19 19.57 0.27
10 2.2 × 106 32.77 2.86 23.00 0.68
1 2.2 × 105 35.18 2.23 26.40 0.95
0.1 2.2 × 104 37.47 1.04 30.19 0.90
0.01 2.2 × 103 - - 33.23 0.84
0.001 2.2 × 102 - - 36.70 1.46
  1. a based on weight of P. aeruginosa PAO1 genome of 4.5 fg
  2. b oprL correlation coefficient r2 = 0.9865, amplification efficiency = 114.84%
  3. c HW2 correlation coefficient r2 = 0.9996, amplification efficiency = 96.84%
  4. d Fluorescent dye used for primer labeling; see Materials and Methods.