Figure 1
From: A diagnostic PCR assay for the detection of an Australian epidemic strain of Pseudomonas aeruginosa
PCR detection of oprL and the AES-I HW2 locus from CF sputum. Sputum from 2 patients that had been shown by PFGE screening to contain either AES-I or a unique strain of P. aeruginosa (UCF) was swabbed onto Whatman FTA® Elute cards. The oprL and HW2 loci were amplified by PCR from these sputum samples (UCF-FTA, AES-I-FTA) or chromosomal DNA (UCF-DNA, AES-I-DNA) purified from the P. aeruginosa strains isolated from the same sputum sample. These strains were also confirmed by PFGE to be AES-I or a unique strain of P. aeruginosa (data not shown).