Table 2 Primers used for the detection of carbapenemase genes.

AmpC

5'- ACTTACTTCAACTCGCGACG -3'

5'- TAAACACCACATATGTTCCG -3'

^blaampC Amplification

Classical PCR (annealing temperature 44°C)

[18]

OXA-51

5'- ATGAACATTAAAGCACTCTTAC -3'

5'- CTATAAAATACCTAATTGTTCT -3'

^blaOXA-51 Amplification

Classical PCR (annealing temperature 50°C)

[19]

OXA-23

5'- GCAAATAM AGAATATGTS CC -3'

5'- CTCM ACCCAR CCR GTCAACC -3'

^blaOXA-23 Amplification & sequencing

Classical PCR (annealing temperature 58°C)

[20]

OXA-24

5'- GGTTAGTTGGCCCCCTTAAA -3'

5'- AGTTGAGCGAAAAGGGGATT -3'

^blaOXA-24 Amplification

Classical PCR (annealing temperature 59°C)

[21]

VIM

5'- GTTTGGTCGCATATCGCAAC -3'

5'- CTACTCAACGACTGAGCGATTTGT -3'

^blaVIM Amplification

Classical PCR (annealing temperature 60°C)

[13]

IMP

5'- CTACCGCAGCAGAGTCTTTG -3'

5'- AACCAGTTTTGCCTTACCAT -3'

^blaIMP

Classical PCR (annealing temperature 50°C)

[13]

IsAba-1 F/OXA-23 R

5'- CACGAATGCAGAAGTTG - 3'

5'-TTAAATAATATTCAGCTGT - 3'

Regulation of OXA-23 by IsAba- 1

Classical PCR (annealing temperature 50°C)

[22]

REP

5'-IIIGCGCCGICATCAGGC-3'

5'-ACGTCTTATCAGGCCTAC-3

REP-PCR Amplification

Classical PCR (annealing temperature 40°C)

[23]