Incubation time (hour) | CFUa | filC-d ampliconsb |
---|
0 | 3 | - - - |
1 | 4 | - - - |
2 | 17 | - - - |
3 | 105 | +++ |
4 | 209 | +++ |
5 | 4461 | +++ |
- *Three bacteria of Salmonella serovar Typhi were incubated in the TSB-bile blood culture containing 2.4% ox bile and 20% blood. a The mean of three independent experiments; b Salmonella serovar Typhi fliC-d amplicons resulting from PCR using the DNA templates prepared from three independent cultures.