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Table 3 Summary of results showing the identification and distribution of antibiotic resistance genes in E. coli isolates from equine faecal samples taken from hospital and livery premises.

From: Antimicrobial resistance in equine faecal Escherichia coli isolates from North West England

Antibiotic(No. of resistant
isolates investigated)
Source and number(N) of isolates Identified by PCR Not identified by
PCR
Antibiotic resistance gene composition tested by PCR (details in table legend)
AMP (191) Hospital n = 177
Livery n = 14
169
5
8
9
TEM & SHV β-lactamase genes
CHL (102) Hospital n = 97
Livery n = 5
75
0
22
5
catI catII catIII cmlA
TET (198) Hospital n = 177
Livery n = 21
154
18
23
3
tetB tetA tetC tetD tetE tetG
TRI (279) Hospital n = 209
Livery n = 70
195
65
14
5
dfrA1 dfrA17 dfrA12 dfrA9 dfrA7, dfrA13
  1. All genes listed were tested by PCR amplification using gene-specific primer pairs listed in Table 1. Genes positively identified by PCR are shown in bold, and are listed according to their frequency of occurrence within each resistance group: AMP, TEM lactamase genes 91%, SHV β-lactamase genes 0.6%; CHL, catI 73.5%; TET, tetB 71%, tetA 18%,tetA+B 11%; TRI, dfrA1 40.3%, dfrA17 28%, dfrA12 17.3%, dfrA9 0.3%. Those genes failed to give rise to detectable levels of PCR product are listed in normal type.
  2. Abbreviations: AMP, ampicillin; CHL, chloramphenicol; TET, tetracycline; TRI, trimethoprim