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Table 2 Amplification conditions.

From: Genotypic detection of rifampicin and isoniazid resistant Mycobacterium tuberculosis strains by DNA sequencing: a randomized trial

Gene

Denaturation

Annealing

Extension

No. of cycles

rpoB

94°C, 1 min.

55°C,1 min.

72°C, 1 min.

40 cycles

Ka G

94°C, 1 min.

61°C, 1 min.

72°C, 1 min.

30 cycles

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Annals of Clinical Microbiology and Antimicrobials

ISSN: 1476-0711

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