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Table 1 Alamar blue MICs and MBICs* of antibiotics against planktonic- and biofilm-grown S. aureus ATCC 29213

From: Application of a high throughput Alamar blue biofilm susceptibility assay to Staphylococcus aureus biofilms

Drug

Planktonic MIC (μg/ml)

Biofilm MBIC (μg/ml)

gentamicin

0.25

>2048

clindamycin

16

>2048

bacitracin

32

>2048

nitrofurazone

8

>256

vancomycin

0.5

>2048

ciprofloxacin

0.25

>128

enrofloxacin

0.0625

>2048

ceftriaxone

2

>2048

penicillin

0.5

>2048

oxacillin

0.0625

>2048

rifampin

<0.0008

>2048

  1. *MIC or MBIC defined as the lowest drug concentration resulting in ≤50% reduction of AB (average of two experiments) and a purple/blue well 60 min after addition of AB. MBICs for nitrofurazone and rifampin were only determined using the ≤50% reduction of AB criterion, as these antibiotics are colored at high doses.