Figure 3

Accumulation studies with ciprofloxacin. The fluorescence of the supernatant was measured with spectroflourimeter (LS 55 Fluorescence Spectrometer, 120 V, Perkin-Elmer model) at an excitation 275 nm and emission 440 nm for ciprofloxacin. The results for six representative MDR strains, namely, AC0003, AC0007, AC0008, AC0050, AC0062, AC0089 and one sensitive strain AC0030 are shown here. The graphs reflect the difference in fluorescence displayed by the bacterial cell in the presence and absence of efflux pump inhibitor CCCP. The arrow indicates the time of addition of CCCP. All experiments were carried out at least three times.