Minor contribution of mutations at iniA codon 501 and embC-embA intergenic region in ethambutol-resistant clinical Mycobacterium tuberculosis isolates in Kuwait

Table 2 Primer sequences and their locations in target genes used for PCR amplification of iniA501 and embC-embA IGR

Primer pair	Primer name	Primer sequence	Target gene^a	Direction^b/position	Target region	Amplicon size (bp)	Mutations detected by
1	INIAF	5'-CGCTGGGCCGGATGGAATCGAA-3'	iniA	F, 1403–1424	Codon 501	241	Hpy 99 I RFLP
	INIAR	5'-ACGAAGCGCCGCACATTGGCCTT-3'		R, 1643–1621
2	IGRF	5'-GTTACAGCGGTTGACGCCTTACTA-3'	IGR^c	F, 29061–29084	-11 to -43^d	261	Sequencing
	IGRR	5'-CGATTCCCGAGACGACGGCTGCTA-3'		R, 29321–29298

^aThe accession numbers for target genes are iniA, Z95324; embC-embA IGR, AL123456
^bF, forward; R, reverse
^cIGR, he embC-embA intergenic region
^d-11 to -43 corresponds to -11, -12, -16 and -43 positions (relative to the start codon of embA) in embC-embA IGR

ISSN: 1476-0711