Verification of the ProPneumo-1 assay for the simultaneous detection of Mycoplasma pneumoniae and Chlamydophila pneumoniae in clinical respiratory specimens

Table 3 Overview of test results for a subset of C. pneumoniae patients

Patient No	Sex	Age	Specimen	Pathogen	Monoplex PCR	Prodesse PP-1	Seegene PB-1
01	M	13	BAL	Cpn	+	+	+
02	M	0.2	NPS	Cpn	+	+	+
03	F	10	BAL	Cpn	+	+	+
04	F	10	ETA	Cpn	+	+	+
05	F	2	BAL	Cpn	+	+	+
06	F	10	BAL	Cpn	+	+	+
07	F	63	NPS	Cpn	+	+	+
08	M	32	NPS	Cpn	+	+	+
09	F	63	TS	Cpn	+	+	+
10	F	19	NA	Cpn	+	+	+
11	M	48	BAL	Cpn	+	-	-
12	NA	NA	Autopsy	Cpn	-	+	+
13	NA	NA	Sputum	Cpn	-	+	+
**14	F	25	NPS			+	+
**15	F	44	NPS			+	+

Abbreviations: NA, not available; M, male; F, Female; NPS, nasopharyngeal swab; NPA, nasopharyngeal aspirate; BAL, bronchoalveolar lavage; ETA, endotracheal aspirate; TS, throat swab; BR, bronchial; Cpn, C. pneumoniae; Mpn, M. pneumoniae; PP-1, ProPneumo-1; PB-1, PneumoBacter-1. *, These specimens tested negative for C. pneumoniae in the monoplex assay but were defined positive for M. pneumoniae by the ProPneumo-1 and PneumoBacter-1 assays. **, These specimens tested negative for M. pneumoniae in culture but were defined positive for C. pneumoniae by the ProPneumo-1 and PneumoBacter-1 assays.

ISSN: 1476-0711