Skip to main content

Table 2 Suspension test for validation of neutralization of chlorhexidine digluconate.

From: Pitfalls in efficacy testing – how important is the validation of neutralization of chlorhexidine digluconate?

Storage
time
Test organism exposed to
(name of the test)
Staphylococcus
aureus
Staphylococcus
epidermidis
Micrococcus
luteus
Corynebacterium
jeikeium
Candida
albicans
<1 min. neutralized antiseptic solution
(neutralizer toxicity test)
1.82 ± 0.04 1.77 ± 0.03 0* 1.66 ± 0.09 1.06 ± 0.12*
  saline peptone water
(organism viability test)
1.90 ± 0.05 1.84 ± 0.07 1.74 ± 0.03 1.79 ± 0.02 2.02 ± 0.03
3 h neutralized antiseptic solution
(neutralizer toxicity test)
1.80 ± 0.02 0.86 ± 0.14* 0* 1.44 ± 0.22* 0.13 ± 0.23*
  saline peptone water
(organism viability test)
1.88 ± 0.06 1.81 ± 0.02 1.73 ± 0.05 1.77 ± 0.08 2.03 ± 0.02
  1. Mean and SD of the number of CFU (log10) when exposed to the neutralized antiseptic solution or saline peptone water at storage times of < 1 min and 3 h; * significant difference (p < 0.05) in comparison to organism viability test, Tukey-HSD