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Table 2 Suspension test for validation of neutralization of chlorhexidine digluconate.

From: Pitfalls in efficacy testing – how important is the validation of neutralization of chlorhexidine digluconate?

Storage

time

Test organism exposed to

(name of the test)

Staphylococcus

aureus

Staphylococcus

epidermidis

Micrococcus

luteus

Corynebacterium

jeikeium

Candida

albicans

<1 min.

neutralized antiseptic solution

(neutralizer toxicity test)

1.82 ± 0.04

1.77 ± 0.03

0*

1.66 ± 0.09

1.06 ± 0.12*

 

saline peptone water

(organism viability test)

1.90 ± 0.05

1.84 ± 0.07

1.74 ± 0.03

1.79 ± 0.02

2.02 ± 0.03

3 h

neutralized antiseptic solution

(neutralizer toxicity test)

1.80 ± 0.02

0.86 ± 0.14*

0*

1.44 ± 0.22*

0.13 ± 0.23*

 

saline peptone water

(organism viability test)

1.88 ± 0.06

1.81 ± 0.02

1.73 ± 0.05

1.77 ± 0.08

2.03 ± 0.02

  1. Mean and SD of the number of CFU (log10) when exposed to the neutralized antiseptic solution or saline peptone water at storage times of < 1 min and 3 h; * significant difference (p < 0.05) in comparison to organism viability test, Tukey-HSD