Fatal bacteremia due to immotile Vibrio cholerae serogroup O21 in Vientiane, Laos – a case report

Table 1 Summary of the gene regions detected in the patients isolates from ascitic fluid and blood culture compared to the 569B reference organism and serogroup O21 609-68 and 418-03 strains kindly provided by the National Institute of Infectious Diseases, Tokyo, Japan.

Gene	Primer	Product length/bp	Inaba 569B	609-68 India	418-03 USA	Patient's ascitic fluid	Patient's blood culture
ompW	ompW 1/2	588	+	+	+	+	+
ompW	ompW1/4	304	+	+	+	+	+
ompW	ompW 2/4	336	+	+	+	+	+
ompW	ompW F/R	373	+	-	-	-	-
ToxR	Tox F/R	337	+	+	+	+	+
ctxA	ctxA F/R	354	+	-	-	-	-
ompU	ompU F/R	283	+	-	-	-	-
ompK	ompK F/R	310	+	-	-	-	-
TCP	TCP F/R	265	+	-	-	-	-

Notes. Amplification by was done in a 25 μl reaction mixture containing 3 μl of template DNA, 200 μM of each dNTP, 1X reaction buffer, 1.25 units of Taq polymerase (TaKaRa Ex-Taq), and 0.5 μM of each primers. The initial amplification conditions were one cycle at 95°C for 7 minutes, 35 cycles at 95°C for 30 seconds. 55°C for 30 seconds, and 72°C for 30 seconds; and elongation step of 72°C for 5 minutes. Nested PCR was performed with the same method except that the annealing temperature was 60°C and the template was 2 μl of the first PCR product. Electrophoresis was performed on a 1.8% agarose gel.

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