Figure 2
Direct inactivation of different concentrations of A. arborescens essential oil on HSV-1 at various times as determined by plaque reduction assay. HSV-1 was mixed with A. arborescens essential oil and incubated for 15 min. (black circle), 1 h (black square) or 2 h (white circle) at 37°C. IC50 of 2.4 μg/ml as determined after 1 h pre-incubation at 37°C, shifted to 1.14 μg/ml and 6.9 μg/ml when HSV-1 was pre-incubated for 2 h or 15 min, respectively. Results are presented as mean percentage of control of three separate experiments.