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Figure 6 | Annals of Clinical Microbiology and Antimicrobials

Figure 6

From: β-lactam antibiotic-induced release of lipoteichoic acid from Staphylococcus aureus leads to activation of neutrophil granulocytes

Figure 6

LTA enhances the phagocytic activity of PMN. Neutrophil granulocytes were incubated for 75 minutes at 37°C in medium alone or in the presence of LTA (10 μg/ml) or in the presence of a supernatant of flucloxacillin (20 × MIC)-exposed S. aureus. Subsequently, viable S. aureus (3:1; bacteria:PMN) of three strains (the reference strain DSM 20233 and two clinical isolates) were added and the cells incubated for an additional 30 minutes at 37°C. The number of PMN with phagocytosed S. aureus was assessed on Giemsa-stained cytocentrifuge slides under oil immersion light microscopy. Data (mean ± SD) of three independent experiments are shown. Significant differences (ANOVA, Dunnets test) to unstimulated PMN are indicated (*)

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