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Table 2 Additional mutations acquired in 26 randomly selected colonies of rifampicin resistant isolate R190 (S522L) after a second round of selection with rifabutin.

From: Acquisition of rifabutin resistance by a rifampicin resistant mutant of Mycobacterium tuberculosis involves an unusual spectrum of mutations and elevated frequency

Strain

Conc. of RFB used for selection μg/ml

Additional mutations identified* codons 505–531 (Cluster I 507–533)

Additional mutations identified* codons 144–148

1

0.8

F505L TTC>TTA

None

2

0.8

F505L TTC>TTG

None

3

0.8

S512G AGC>GGC

None

4

8

S512G AGC>GGC

None

5

8

S512G AGC>GGC

None

6

8

S512G AGC>GGC

None

7

0.8

H526Y CAC>TAC

None

8

8

H526Y CAC>TAC

None

9

0.8

S531L TCG>TTG

None

10

0.8

S531L TCG>TTG

None

11

0.8

S531L TCG>TTG

None

12

0.8

S531L TCG>TTG

None

13

8

None

V144M GTG>ATG

14

8

None

V144M GTG>ATG

15

8

None

V144G GTG>GGG

16

0.8

None

V146F GTC>TTC

17

0.8

None

V146F GTC>TTC

18

0.8

None

V146F GTC>TTC

19

8

None

V146F GTC>TTC

20

8

None

V146F GTC>TTC

21

8

None

V146F GTC>TTC

22

8

None

Q148H CAG>CAC

23

0.8

None

Q148H CAG>CAC

24

0.8

None

Q148H CAG>CAC

25

0.8

None

None

26

0.8

None

None

  1. *All isolates retained the original S522L TCG>TTG mutation from their R190 parent and had at most a single additional mutation identified. Codons are numbered according to the E. coli numbering system.