Development of a Multiple-Locus Variable number of tandem repeat Analysis (MLVA) for Leptospira interrogans and its application to Leptospira interrogans serovar Australis isolates from Far North Queensland, Australia

Table 3 Characteristic of the Six VNTR loci

Loci	Repeat Motif	Repeat size (bp)^a	Total Flanking Regions (bp)^a	Repeat range (min-max)	No. of alleles	Diversity (D)
8	GGAAAACTCAACACAA CGCTCTTTATGAATCG CGTT	36	124	0–16	14	0.88
27	TTGTGGGAACTCTTAC AATTTGAGATTTTACA GTAAAACTTGGAAGTT GTGGGAACTCTTACAA TTTGAGATTTTACAGTA AAACTTGGAAATTGTG GGAACTCTTACAACTT GAGATTTTACAGTGGG ACTTTGAAG	138	183	0–4	6	0.80
29	GATTTTACAGTTAGAC TTTGAAATTGTGGGAA CTCCCACGGATTTGG	47	90	0–17	14	0.92
30	TCCCACATATTCAAGA TTAAACTGTAAAATTGT GATTTGTGGTAGT	46	228	0–12	12	0.88
36	CTTAGACTTTGTGTGA GTTCCCACATTTTAAA GTAAAA	38	161	0–52	19	0.93
50	AAAATGTAGGAACTAC CACAAACACTGACTTT ACAGATAAATTCTC	46	106	012	9	0.83

ISSN: 1476-0711