Development of a Multiple-Locus Variable number of tandem repeat Analysis (MLVA) for Leptospira interrogans and its application to Leptospira interrogans serovar Australis isolates from Far North Queensland, Australia

Table 2 PCR primers used in Study

Primer Name	Direction	Sequence (5'-3')	TheoreticalMelting Temperature (°C)
V8	Forward	CAA GTG TTC GAC AAG AAT GAG	57.4
	Reverse	CTC ACC GGT AGA ACG CTC TTT T	58.4
V27	Forward	TCG TCG GGT GAG CTA AAA TAT	57.0
	Reverse	TTC TTT CGG TGG CAA GG TTT	59.8
V29	Forward	ATC GTT TTG GCA GTT TTT GCT	57.7
	Reverse	CTA GAA AAT TCC GCG TAG GG	57.2
V30	Forward	AAG TAA GAT AGG TTC GGC GTT TA	57.9
	Reverse	ACT TGG GTG TTA ATC GCA AAA	57.7
V36	Forward	TGG TTC TTG GGG TAA TTC TGT T	58.2
	Reverse	CTA CCA GGA GAT TAT CAA AAC GAA	57.9
V50	Forward	CTT GTT GGA TCA CAA TAC GAA CTA TA	58.4
	Reverse	GGTAAGGGACAAAGTAAGTGAAGC	58.9

ISSN: 1476-0711