Mutation at the position 2058 of the 23S rRNA as a cause of macrolide resistance in Streptococcus pyogenes

Jalava, Jari; Vaara, Martti; Huovinen, Pentti

doi:10.1186/1476-0711-3-5

Annals of Clinical Microbiology and Antimicrobials

Table 1 PCR and sequencing primers

From: Mutation at the position 2058 of the 23S rRNA as a cause of macrolide resistance in Streptococcus pyogenes

Primer	Target gene	Position	Use	Sequence (5'-3')	Reference
23Scom ^a	23S rRNA	6-26^b	PCR/Seq	AGTTAATAAGGGCGCACGGTG	This work
23Sg1 ^c	23S rRNA gene1	1577119-157741^d	PCR/Seq	AAGACGTATTGAAGCTTACTCTA	This work
23Sg2 ^c	23S rRNA gene2	22879-22857 ^d	PCR/Seq	AGCTACTTCCCGAACTGATGCAC	This work
23Sg3 ^c	23S rRNA gene3	29128-29106 ^d	PCR/Seq	GATAACCGTCTTCTTTCCCCTTT	This work
23Sg4 ^c	23S rRNA gene4	85192-85170 ^d	PCR/Seq	CATATTTCTAACACGGGCAGTAG	This work
23Sg5 ^c	23S rRNA gene5	270607-270582 ^d	PCR/Seq	CACTGCCAAGCTATCTAAACGTA	This work
23Sg6 ^c	23S rRNA gene6	1330037-1330059^d	PCR/Seq	CAATTGAATAGCCTGCACGTTCG	This work
MS2 ^e	23S rRNA	550-573^b	Sequencing	CGTGTGCCTACAACAAGTTCGAGC	This work
MS3 ^e	23S rRNA	573-550^b	Sequencing	GCTCGAACTTGTTGTAGGCACACG	This work
MS4 ^e	23S rRNA	1016-997^b	Sequencing	TTTCGACTACGGATCTTAGC	[11]
MS5 ^e	23S rRNA	867-885^b	Sequencing	CTGTTTGGGTGAGGGGTCC	[11]
MS6 ^e	23S rRNA	1424-1446^b	Sequencing	GGACGCAGTAGGCTAACTAAACC	This work
MS7 ^e	23S rRNA	1446-1424^b	Sequencing	GCTTTAGTTAGCCTACTGCGTCC	This work
MS8 ^e	23S rRNA	2021-2040^b	Sequencing	ACCTGCATCTTCACAGGTAC	[11]
MS9 ^e	23S rRNA	1902-1921^b	Sequencing	CGGCGGCCGTAACTATAACG	[11]
MS10 ^e	23S rRNA	2415-2437^b	Sequencing	GCCATCGCTCAACGGATAAAAGC	This work
MS11 ^e	23S rRNA	2437-2415^b	Sequencing	GCTTTTATCCGTTGAGCGATGGC	This work
MS12 ^e	23S rRNA	2904-2880^b	Sequencing	TTGGATAAGTCCTCGAGCTATTAG	[11]
L4fu	L4	64481-64498^c	PCR/Seq	GCAAACACAGCTCCTAAG	This work
L4rd	L4	65751-65734^c	PCR/Seq	CTGTGTAACGACCAACGC	This work
L4f156	L4	65074-65091^c	Sequencing	AACCGTTCAGCAGTATCA	This work
L4r444	L4	65307-65290^c	Sequencing	TGAAAGGCCTTCTACAGC	This work
L22-1	L22	67079-67098^c	PCR/Seq	GCAGACGACAAGAAAACACG	[11]
L22-2	L22	67500-67481^c	PCR/Seq	ATTGGATGTACTTTTTGACC	[11]

^a A PCR primer common to all 23S rRNA genes ^b Position in the 23S rRNA molecule (E. coli accession number J01695, numbering). ^c rRNA operon spesific PCR primers, artificial numbering of the 23S rRNA genes of the S. pyogenes ^d Position in the S. pyogenes genome (accession number AE004092). ^e23S rRNA gene sequencing primers

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ISSN: 1476-0711

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