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Table 1 Oligonucletoides and optimised experimental PCR amplification conditions

From: Early detection of Pseudomonas aeruginosa – comparison of conventional versus molecular (PCR) detection directly from adult patients with cystic fibrosis (CF)

Target

Gene locus

Primer 5'-----------------------3'

Optimum MgCl2 concentration (mM)

Annealing temperature

Size of Amplicon (bp)

Eubacteria [7]

16S rRNA

f: 5'-AGG ATT AGA TAC CCT GGT AGT CCA-3'

2.5

55°C

312

  

r: 5'-ACT TAA CCC AAC ATC TCA CGA CAC-3'

   

Pseudomonas aeruginosa [8]

opr L1

f: 5'-ATG GAA ATG CTG AAA TTC GGC-3'

2.5

55°C

504

  

r: 5'-CTT CTT CAG CTC GAC GCG ACG-3'

   

Pseudomonas aeruginosa [9]

exo A2

f: 5'-GAC AAC GCC CTC AGC ATC ACC AGC-3'

2.5

72°C

396

  

r: 5'-CGC TGG CCC ATT CGC TCC AGC GCT-3'

   
  1. 1, outer membrane lipoprotein; 2, exotoxin A.
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Annals of Clinical Microbiology and Antimicrobials

ISSN: 1476-0711

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