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Table 1 Oligonucletoides and optimised experimental PCR amplification conditions

From: Early detection of Pseudomonas aeruginosa – comparison of conventional versus molecular (PCR) detection directly from adult patients with cystic fibrosis (CF)

Target Gene locus Primer 5'-----------------------3' Optimum MgCl2 concentration (mM) Annealing temperature Size of Amplicon (bp)
Eubacteria [7] 16S rRNA f: 5'-AGG ATT AGA TAC CCT GGT AGT CCA-3' 2.5 55°C 312
   r: 5'-ACT TAA CCC AAC ATC TCA CGA CAC-3'    
Pseudomonas aeruginosa [8] opr L1 f: 5'-ATG GAA ATG CTG AAA TTC GGC-3' 2.5 55°C 504
   r: 5'-CTT CTT CAG CTC GAC GCG ACG-3'    
Pseudomonas aeruginosa [9] exo A2 f: 5'-GAC AAC GCC CTC AGC ATC ACC AGC-3' 2.5 72°C 396
   r: 5'-CGC TGG CCC ATT CGC TCC AGC GCT-3'    
  1. 1, outer membrane lipoprotein; 2, exotoxin A.