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Table 3 Minimal inhibitory concentrations (MIC) for E. coli BL21 Star (DE3) transformants expressing LinF or AadA2.

From: Prevalence and characterization of integrons in blood culture Enterobacteriaceae and gastrointestinal Escherichia coli in Norway and reporting of a novel class 1 integron-located lincosamide resistance gene

Vector construct

MIC (μg/ml)

 

Lincomycin

Clindamycin

Streptomycin

Kanamycin

Gentamicin

PET101X38 (linF)1

512

128

16

8

4

PET101A17 (aadA2)2

128

64

256

8

4

PET101C (control)3

128

64

16

8

4

  1. 1 linF cloned into vector pET101 2 aadA2 cloned into vector pET101 3 Control PCR product (pETdirectional TOPO Expression kit, Invitrogen) cloned into vector pET101
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Annals of Clinical Microbiology and Antimicrobials

ISSN: 1476-0711

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