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Table 4 The sensitivity and specificity of the Real-GP®, -GN®, and -CAN® real-time PCR assay with positive and negative blood culture bottle samples

From: Real-time PCR TaqMan assay for rapid screening of bloodstream infection

Blood culture Real-time PCR Sensitivity Specificity
TaqMan assay (n)
Positive Negative
Blood culture positive (n = 256) 255 1 99.6% -
Gram-positive bacteria (176) 175 1 99.4% -
Gram-negative bacteria (70) 70 0 100% -
Candida species (4) 4 0 100% -
Multiple infection (6) 6 0 100% -
Blood culture negative (n = 200) 21 179 - 89.5%
Gram-positive bacteria 7 193 - 96.5%
Gram-negative bacteria 14 186 - 93.0%
Candida species 0 200 - 100%