Table 3 Comparison of the results of Real-GP®, -GN®, and -CAN® real-time PCR assay and BACTEC 9240 for detection of bloodstream infection in positive and negative blood culture bottle samples
From: Real-time PCR TaqMan assay for rapid screening of bloodstream infection
Blood culture result | Real-time PCR TaqMan assay (n) | GP | GN | CAN | |
|---|---|---|---|---|---|
Positive | Negative | (CTrange) | (CTrange) | (CTrange) | |
Blood culture positive (n = 256) | 255 | 1 | |||
Gram-positive bacteria (n = 176) | 175 | 1 | |||
Staphylococccus epidermidis (47) | 47 | 0 | 13.08-33.99 | UD | UD |
S. aureus (24) | 24 | 0 | 13.24-34.19 | UD | UD |
S. hominis (17) | 16 | 1 | 13.69-30.00 | UD | UD |
S. capitis (14) | 14 | 0 | 13.60-25.10 | UD | UD |
S. haemolyticus (8) | 8 | 0 | 14.68-33.30 | UD | UD |
S. warneri (1) | 1 | 0 | 18.50 | UD | UD |
S. saprophyticus (1) | 1 | 0 | 16.57 | UD | UD |
S. xylosus (1) | 1 | 0 | 21.23 | UD | UD |
S. chleiferi (1) | 1 | 0 | 20.44 | UD | UD |
Streptococcus salivarius (5) | 5 | 0 | 13.55-23.58 | UD | UD |
S. mitis (4) | 4 | 0 | 11.52-23.12 | UD | UD |
S. pneumoniae (4) | 4 | 0 | 16.37-17.77 | UD | UD |
S. agalactiae (2) | 2 | 0 | 21.52-25.95 | UD | UD |
S. pyogenes (1) | 1 | 0 | 22.10 | UD | UD |
S. dysgalactiae (1) | 1 | 0 | 15.81 | UD | UD |
S. parasangus (1) | 1 | 0 | 12.96 | UD | UD |
Streptococcus spp. (2) | 2 | 0 | 17.87-24.89 | UD | UD |
Enterococcus faecium (8) | 8 | 0 | 26.43-27.54 | UD | UD |
E. faecalis (1) | 1 | 0 | 14.50 | UD | UD |
Micrococcus spp. (5) | 5 | 0 | 20.96-31.33 | UD | UD |
Propionibacterium acnes (3) | 3 | 0 | 23.77-26.86 | UD | UD |
Peptostreptococcus asaccharolyticus (1) | 1 | 0 | 26.72 | UD | UD |
Peptostreptococcus micros (1) | 1 | 0 | 26.00 | UD | UD |
Corynebacterium spp. (6) | 6 | 0 | 26.47 | UD | UD |
Gram positive rods (16) | 16 | 0 | 12.43-34.39 | UD | UD |
Gram-negative bacteria (n = 70) | 70 | 0 | |||
Escherichia coli (35) | 35 | 0 | UD | 9.86-21.89 | UD |
Klebsiella pneumoniae (13) | 13 | 0 | UD | 12.84-13.20 | UD |
Acinetobacter baumannii (5) | 5 | 0 | UD | 10.53-11.89 | UD |
A. woffii (1) | 1 | 0 | UD | 12.22-15.11 | UD |
Enterobacter spp. (2) | 2 | 0 | UD | 6.56 | UD |
Pseudomonas aeruginosa (3) | 3 | 0 | UD | 12.08 | UD |
Salmonella group D (1) | 1 | 0 | UD | 13.62-21.48 | UD |
Proteus mirabilis (1) | 1 | 0 | UD | 14.38 | UD |
Aeromonas spp. (2) | 2 | 0 | UD | 20.10 | UD |
Morganella morganii (1) | 1 | 0 | UD | 10.39 | UD |
Haemophillus influenzae (1) | 1 | 0 | UD | 20.68 | UD |
Chryseobacterium indologenes (1) | 1 | 0 | UD | 14.40 | UD |
Sphingomonas paucimobilis (1) | 1 | 0 | UD | 24.08 | UD |
Serratia marcescens (1) | 1 | 0 | UD | 9.48 | UD |
Citrobacter freundii (1) | 1 | 0 | UD | 14.24 | UD |
Candida species (n = 4) | 4 | 0 | |||
Candida albicans (2) | 2 | 0 | UD | UD | 26.98-31.52 |
C. parapsilosis (1) | 1 | 0 | UD | UD | 31.98 |
C. tropicalis (1) | 1 | 0 | UD | UD | 22.81 |
* Polymicrobial infection (n = 6) | 6 | 0 | |||
Streptococcus agalactiae, Citrobacter koseri (1) | 1 | - | 14.61 | 10.61 | UD |
Enterococcus faecium, Candida albicans (1) | 1 | - | 17.18 | UD | 29.51 |
Enterococcus faecalis, Proteus mirabilis (1) | 1 | - | UD | 12.14 | UD |
Escherichia coli, Enterococcus gallinarym (1) | 1 | - | UD | 13.25 | UD |
Klebsiella pneumonia, Enterococcus casseliflavus (1) | 1 | - | UD | 13.31 | UD |
Klebsiella pneumonia, Enterobacter cloacae (1) | 1 | - | UD | 14.42 | UD |
Blood culture negative (n = 200) | 21 | 179 | |||
Gram-positive bacteria | 7 | 193 | 17.54-27.43 | - | - |
Gram-negative bacteria | 14 | 186 | - | 20.48-31.26 | - |
Candida species | 0 | 200 | - | - | - |