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Table 3 Comparison of the results of Real-GP®, -GN®, and -CAN® real-time PCR assay and BACTEC 9240 for detection of bloodstream infection in positive and negative blood culture bottle samples

From: Real-time PCR TaqMan assay for rapid screening of bloodstream infection

Blood culture result Real-time PCR TaqMan assay (n) GP GN CAN
Positive Negative (CTrange) (CTrange) (CTrange)
Blood culture positive (n = 256) 255 1    
Gram-positive bacteria (n = 176) 175 1    
Staphylococccus epidermidis (47) 47 0 13.08-33.99 UD UD
S. aureus (24) 24 0 13.24-34.19 UD UD
S. hominis (17) 16 1 13.69-30.00 UD UD
S. capitis (14) 14 0 13.60-25.10 UD UD
S. haemolyticus (8) 8 0 14.68-33.30 UD UD
S. warneri (1) 1 0 18.50 UD UD
S. saprophyticus (1) 1 0 16.57 UD UD
S. xylosus (1) 1 0 21.23 UD UD
S. chleiferi (1) 1 0 20.44 UD UD
Streptococcus salivarius (5) 5 0 13.55-23.58 UD UD
S. mitis (4) 4 0 11.52-23.12 UD UD
S. pneumoniae (4) 4 0 16.37-17.77 UD UD
S. agalactiae (2) 2 0 21.52-25.95 UD UD
S. pyogenes (1) 1 0 22.10 UD UD
S. dysgalactiae (1) 1 0 15.81 UD UD
S. parasangus (1) 1 0 12.96 UD UD
Streptococcus spp. (2) 2 0 17.87-24.89 UD UD
Enterococcus faecium (8) 8 0 26.43-27.54 UD UD
E. faecalis (1) 1 0 14.50 UD UD
Micrococcus spp. (5) 5 0 20.96-31.33 UD UD
Propionibacterium acnes (3) 3 0 23.77-26.86 UD UD
Peptostreptococcus asaccharolyticus (1) 1 0 26.72 UD UD
Peptostreptococcus micros (1) 1 0 26.00 UD UD
Corynebacterium spp. (6) 6 0 26.47 UD UD
Gram positive rods (16) 16 0 12.43-34.39 UD UD
Gram-negative bacteria (n = 70) 70 0    
Escherichia coli (35) 35 0 UD 9.86-21.89 UD
Klebsiella pneumoniae (13) 13 0 UD 12.84-13.20 UD
Acinetobacter baumannii (5) 5 0 UD 10.53-11.89 UD
A. woffii (1) 1 0 UD 12.22-15.11 UD
Enterobacter spp. (2) 2 0 UD 6.56 UD
Pseudomonas aeruginosa (3) 3 0 UD 12.08 UD
Salmonella group D (1) 1 0 UD 13.62-21.48 UD
Proteus mirabilis (1) 1 0 UD 14.38 UD
Aeromonas spp. (2) 2 0 UD 20.10 UD
Morganella morganii (1) 1 0 UD 10.39 UD
Haemophillus influenzae (1) 1 0 UD 20.68 UD
Chryseobacterium indologenes (1) 1 0 UD 14.40 UD
Sphingomonas paucimobilis (1) 1 0 UD 24.08 UD
Serratia marcescens (1) 1 0 UD 9.48 UD
Citrobacter freundii (1) 1 0 UD 14.24 UD
Candida species (n = 4) 4 0    
Candida albicans (2) 2 0 UD UD 26.98-31.52
C. parapsilosis (1) 1 0 UD UD 31.98
C. tropicalis (1) 1 0 UD UD 22.81
* Polymicrobial infection (n = 6) 6 0    
Streptococcus agalactiae, Citrobacter koseri (1) 1 - 14.61 10.61 UD
Enterococcus faecium, Candida albicans (1) 1 - 17.18 UD 29.51
Enterococcus faecalis, Proteus mirabilis (1) 1 - UD 12.14 UD
Escherichia coli, Enterococcus gallinarym (1) 1 - UD 13.25 UD
Klebsiella pneumonia, Enterococcus casseliflavus (1) 1 - UD 13.31 UD
Klebsiella pneumonia, Enterobacter cloacae (1) 1 - UD 14.42 UD
Blood culture negative (n = 200) 21 179    
Gram-positive bacteria 7 193 17.54-27.43 - -
Gram-negative bacteria 14 186 - 20.48-31.26 -
Candida species 0 200 - - -
  1. *GP-, GN-bacteria or fungi mixed infection.