Table 2 Real-GP®, -GN®, and -CAN® real-time PCR assay results for discriminating the Gram-positive and -negative bacteria and Candida species in 115 clinical isolates
From: Real-time PCR TaqMan assay for rapid screening of bloodstream infection
Culture identification | No. of samples (n) | Real-time PCR TaqMan assay | ||
|---|---|---|---|---|
GP/GN or Candida | Ranged CTvalue | Mean CTvalue | ||
Staphylococcus aureus | 12 | GP | 22.44-26.65 | 24.47 |
Staphylococcus spp. (CoNS) | 8 | GP | 19.46-28.71 | 21.5 |
Streptococcus spp. | 5 | GP | 17.35-30.46 | 24.25 |
Enterococcus faecalis | 4 | GP | 25.2-27.3 | 26.37 |
Enterococcus faecium | 10 | GP | 21.3-31.6 | 26.58 |
Enterococcus mundtii | 1 | GP | 27.85 | 27.85 |
Corynebacterium spp. | 1 | GP | 24.51 | 24.51 |
Escherichia coli | 16 | GN | 12.68-30.65 | 23.26 |
Klebsiella pneumoniae | 13 | GN | 15.48-26.08 | 20.73 |
Pseudomonas aeruginosa | 13 | GN | 15.23-19.96 | 18.11 |
Acinetobacter baumannii | 11 | GN | 18.09-24.65 | 21.04 |
Enterobacter asburiae | 2 | GN | 15.79 | 15.79 |
Enterocobacter cloacae | 1 | GN | 15.41 | 15.41 |
Moraxella catarrhalis | 1 | GN | 33.54 | 33.54 |
Serratia marcescens | 1 | GN | 21.64 | 21.64 |
Providencia rettgeri | 1 | GN | 24.3 | 24.30 |
Morganella morganii | 1 | GN | 20.6 | 20.60 |
Proteus mirabilis | 1 | GN | 24.88 | 24.88 |
Aeromonas spp. | 1 | GN | 25.97 | 25.97 |
Citrobacter fruendii | 2 | GN | 17.11-18.01 | 17.56 |
Candida albicans | 5 | CAN | 17.61-29.56 | 23.9 |
Candida parapsilosis | 3 | CAN | 24.73-30.95 | 27.58 |
Candida tropicalis | 1 | CAN | 26.62 | 26.62 |
Candida glabrata | 1 | CAN | 17.68 | 17.68 |
Total | 115 | |||