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Table 2 Real-GP®, -GN®, and -CAN® real-time PCR assay results for discriminating the Gram-positive and -negative bacteria and Candida species in 115 clinical isolates

From: Real-time PCR TaqMan assay for rapid screening of bloodstream infection

Culture identification No. of samples (n) Real-time PCR TaqMan assay
GP/GN or Candida Ranged CTvalue Mean CTvalue
Staphylococcus aureus 12 GP 22.44-26.65 24.47
Staphylococcus spp. (CoNS) 8 GP 19.46-28.71 21.5
Streptococcus spp. 5 GP 17.35-30.46 24.25
Enterococcus faecalis 4 GP 25.2-27.3 26.37
Enterococcus faecium 10 GP 21.3-31.6 26.58
Enterococcus mundtii 1 GP 27.85 27.85
Corynebacterium spp. 1 GP 24.51 24.51
Escherichia coli 16 GN 12.68-30.65 23.26
Klebsiella pneumoniae 13 GN 15.48-26.08 20.73
Pseudomonas aeruginosa 13 GN 15.23-19.96 18.11
Acinetobacter baumannii 11 GN 18.09-24.65 21.04
Enterobacter asburiae 2 GN 15.79 15.79
Enterocobacter cloacae 1 GN 15.41 15.41
Moraxella catarrhalis 1 GN 33.54 33.54
Serratia marcescens 1 GN 21.64 21.64
Providencia rettgeri 1 GN 24.3 24.30
Morganella morganii 1 GN 20.6 20.60
Proteus mirabilis 1 GN 24.88 24.88
Aeromonas spp. 1 GN 25.97 25.97
Citrobacter fruendii 2 GN 17.11-18.01 17.56
Candida albicans 5 CAN 17.61-29.56 23.9
Candida parapsilosis 3 CAN 24.73-30.95 27.58
Candida tropicalis 1 CAN 26.62 26.62
Candida glabrata 1 CAN 17.68 17.68
Total 115