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Figure 1 | Annals of Clinical Microbiology and Antimicrobials

Figure 1

From: Real-time PCR TaqMan assay for rapid screening of bloodstream infection

Figure 1

Detection limits of the three target probes from 10-fold serial diluted spiked samples. Serially diluted DNA amounts ranging from 108 to 10° CFU/mL were used to determine the detection limit of the multiplex real-time PCR assay. (A) amplification curve of GP-bacteria probe using Staphylococcus aureus, (B) amplification curve of GN-bacteria probe using Escherichia coli, (C) amplification curve of Candida probe using Candida glabrata. The overall detection limit of this assay for GP-, GN-bacteria, and Candida probe was approximately 103 to 104 CFU/mL.

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