Figure 1

Detection limits of the three target probes from 10-fold serial diluted spiked samples. Serially diluted DNA amounts ranging from 10⁸ to 10° CFU/mL were used to determine the detection limit of the multiplex real-time PCR assay. (A) amplification curve of GP-bacteria probe using Staphylococcus aureus, (B) amplification curve of GN-bacteria probe using Escherichia coli, (C) amplification curve of Candida probe using Candida glabrata. The overall detection limit of this assay for GP-, GN-bacteria, and Candida probe was approximately 10³ to 10⁴ CFU/mL.