Table 2 Results of the second phase of the animal study

Castrated

No

No

Yes

Yes

Yes

Treatment

None

Testosterone

None

Testosterone

Estradiol

Infection

Yes

Yes

Yes

Yes

Yes

Colony patterns:

Heart blood

Liver

Heart blood

Liver

Heart blood

Liver

Heart blood

Liver

Heart blood

Liver

None

0

2

1

1

1

1

1

1

1

0

1-10 colonies

2

1

4

3

2

1

0

0

4

3

Dense, but separate

2

2

0

2

0

2

1

2

1

3

Confluent

2

1

1

0

2

1

4

3

0

0

Sample number for lab tests

6

6

5

6

5

WBC (×10⁹/l)

10.70 ± 4.18

5.97 ± 3.22

8.26 ± 4.62

3.61 ± 2.14

2.03 ± 1.05

1 vs 4,5

Neutrophil (%)

50.02 ± 21.82

54.30 ± 26.95

54.12 ± 19.20

38.40 ± 26.29

53.15 ± 21.23

ns

Lymphocyte (%)

45.20 ± 22.89

37.46 ± 28.09

39.56 ± 16.32

58.22 ± 23.98

41.15 ± 22.52

ns

RBC (×10¹²/l)

6.63 ± 0.32

6.44 ± 0.45

5.99 ± 0.47

5.41 ± 0.86

5.95 ± 0.21

4 vs 1,2

HGB (g/dl)

135.40 ± 4.67

132.40 ± 7.16

120.60 ± 12.46

111.40 ± 16.68

120.75 ± 4.65

4 vs 1,2

HCT (l/l)

0.41 ± 0.02

0.40 ± 0.03

0.36 ± 0.03

0.32 ± 0.05

0.35 ± 0.02

4 vs 1,2; 5 vs 1

PLT (×10⁹/l)

718.80 ± 298.45

865.40 ± 212.51

875.60 ± 170.04

696.60 ± 127.91

440.75 ± 198.01

5 vs 2,3

Total protein (g/l)

47.32 ± 2.02

49.53 ± 2.88

51.00 ± 4.45

46.83 ± 4.24

49.76 ± 3.71

ns

Albumin (g/l)

16.68 ± 1.17

17.55 ± 1.18

18.30 ± 1.59

17.03 ± 2.14

18.76 ± 1.50

ns

Globulin (g/l)

30.63 ± 2.67

31.98 ± 2.18

32.70 ± 4.67

29.80 ± 3.03

31.00 ± 2.53

ns

CRP (mg/dl)

375.30 ± 64.54

371.58 ± 33.56

363.30 ± 23.90

329.42 ± 52.21

149.60 ± 131.71

5 vs 1,2,3,4

Testosterone (nmol/l)

21.62 ± 16.08

54.87 ± 1.55

0.87 ± 0.31

41.82 ± 12.10

0.68 ± 0.00

1 vs 2,3,4,5; 2 vs 3,5; 4 vs 3,5

Estradiol (pg/ml)

113.44 ± 30.62

nd

151.75 ± 13.12

nd

867.40 ± 558.15

5 vs 1,3