Study of the frequency of Clostridium difficile tcdA, tcdB, cdtA and cdtB genes in feces of Calves in south west of Iran

Table 1 Primers for identification of C. difficile tcdA, tcdB, cdtA and cdtB genes

Gene target	Primer name	Sequence (5'–3')	Accession	Amplicon size (bp)
*tcdA*	tcdA-F tcdA-R	AATGATGTTACCTAATGCTCCTTC	KC292125	311 bp
*tcdA*	tcdA-F tcdA-R	AGTAAGTTCCTCCTGCTCCATC	KC292125	311 bp
*tcdB*	tcdB-F tcdB-R	CCAGCTAATACACTTGATGAAAACC	KC292190	432 bp
*tcdB*	tcdB-F tcdB-R	TTTCTTCACCTTCTTCATTTCCT	KC292190	432 bp
*cdtA*	cdtA-F cdtA-R	GGAAGCACTATATTAAAGCAGAAGC	HQ639678	219 bp
*cdtA*	cdtA-F cdtA-R	TCTGGGTTAGGATTATTTACTGGAC	HQ639678	219 bp
*cdtB*	cdtB-F cdtB-R	AAAGTTGATGTCTGATTGGGAAG	HQ639678	608 bp
*cdtB*	cdtB-F cdtB-R	TTTGTTGTTGGTGTCACTTTGTA	HQ639678	608 bp

ISSN: 1476-0711