Table 2 Primers used for detection of uropathogenic Escherichia coli serogroups[35]
Serogroup | Target gene | Primer name | Primer sequence (5’-3’) | Size of product (bp) |
|---|---|---|---|---|
O1 | wzx | wl-14632 | GTGAGCAAAAGTGAAATAAGGAACG | 1098 |
| Â | Â | wl-14633 | CGCTGATACGAATACCATCCTAC | Â |
O6 | wzy | wl-14646 | GGATGACGATGTGATTTTGGCTAAC | 783 |
| Â | Â | wl-14647 | TCTGGGTTTGCTGTGTATGAGGC | Â |
O7 | wzx | wl-14648 | CTATCAAAATACCTCTGCTGGAATC | 610 |
| Â | Â | wl-14649 | TGGCTTCGAGATTAAACCTATTCCT | Â |
O8 | orf469 | wl-14652 | CCAGAGGCATAATCAGAAATAACAG | 448 |
| Â | Â | wl-14653 | GCAGAGTTAGTCAACAAAAGGTCAG | Â |
O16 | wzx | wl-14654 | GGTTTCAATCTCACAGCAACTCAG | 302 |
| Â | Â | wl-14655 | GTTAGAGGGATAATAGCCAAGCGG | Â |
O21 | wzx | wl-14676 | CTGCTGATGTCGCTATTATTGCTG | 209 |
| Â | Â | wl-14677 | TGAAAAAAAGGGAAACAGAAGAGCC | Â |
O75 | wzy | wl-17413 | GAGATATACATGGGGAGGTAGGCT | 511 |
| Â | Â | wl-17414 | ACCCGATAATCATATTCTTCCCAAC | Â |
O2 | wzy | wl-14636 | AGTGAGTTACTTTTTAGCGATGGAC | 770 |
| Â | Â | wl-14637 | AGTTTAGTATGCCCCTGACTTTGAA | Â |
O4 | wzx | wl-14642 | TTGTTGCGATAATGTGCATGTTCC | 664 |
| Â | Â | wl-14643 | AATAATTTGCTATACCCACACCCTC | Â |
O15 | wzy | wl-14672 | TCTTGTTAGAGTCATTGGTGTATCG | 183 |
| Â | Â | wl-14673 | ATAAAACGAGCAAGCACCACACC | Â |
O18 | wzx | wl-14656 | GTTCGGTGGTTGGATTACAGTTAG | 551 |
| Â | Â | wl-14657 | CTACTATCATCCTCACTGACCACG | Â |
O22 | wzx | wl-14660 | TTCATTGTCGCCACTACTTTCCG | 468 |
| Â | Â | wl-14661 | GAAACAGCCCATGACATTACTACG | Â |
O25 | wzy | wl-14666 | AGAGATCCGTCTTTTATTTGTTCGC | 230 |
| Â | Â | wl-14667 | GTTCTGGATACCTAACGCAATACCC | Â |
O83 | wzx | wl-14668 | GTACACCAGGCAAACCTCGAAAG | 362 |
| Â | Â | wl-14669 | TTCTGTAAGCTAATGAATAGGCACC | Â |
E. coli | 16SrRNA | wl-3110 | AGAGTTTGATCMTGGCTCAG | 919 |
| Â | Â | wl-3111 | CCGTCAATTCATTTGAGTTT | Â |