Table 1 Primers and probes used in duplex real-time PCR
Target | Primer/probe name | Sequence(5’-3’) | Position | Amplicon size (bp) |
|---|---|---|---|---|
bla NDM | NDM-F | TTGGCGATCTGGTTTTCC | 137–154 | 195 |
NDM-R | GGTTGATCTCCTGCTTGA | 331–314 | ||
NDM-probe | JOE-TGGCAGCACACTTCCTATCTCG- ECLIPSE | 175–196 | ||
bla KPC | KPC-F | CGCAACTGTAAGTTACCG | 162–179 | 187 |
KPC-R | CATGCCTGTTGTCAGATA | 348–331 | ||
KPC-probe | FAM –CCACTGTGCAGCTCATTCAAGG - ECLIPSE | 196-217 |