Annals of Clinical Microbiology and Antimicrobials

Table 2 Comparison of the detection limits of a dilution series of DNA (after amplification) using conventional PCR and duplex real-time PCR*

From: Development of a panel of seven duplex real-time PCR assays for detecting 13 streptococcal superantigens

Groups	Dilution	Detection limit
Groups	Dilution	Conventional PCR	Duplex real-time PCR	Conventional PCR	Duplex real-time PCR
		SpeA		SpeL
1	10⁰	+	+	+	+
	10^-1	+	+	+	+
	10^-2	+	+	+	+
	10^-3	+	+	-	+
	10^-4	-	+	-	+
		SpeB		Ssa
2	10⁰	+	+	+	+
	10^-1	+	+	+	+
	10^-2	-	+	+	+
	10^-3	-	+	-	+
	10^-4	-	-	-	+
		SpeC		SpeH
3	10⁰	+	+	+	+
	10^-1	+	+	+	+
	10^-2	+	+	-	+
	10^-3	-	+	-	+
	10^-4	-	-	-	-
		SpeG		SpeF
4	10⁰	+	+	+	+
	10^-1	+	+	+	+
	10^-2	+	+	+	+
	10^-3	-	+	-	+
	10^-4	-	-	-	+
		SpeM		SpeI
5	10⁰	+	+	+	+
	10^-1	+	+	+	+
	10^-2	+	+	-	+
	10^-3	-	+	-	+
	10^-4	-	-	-	-
		SpeJ		SmeZ
6	10⁰	+	+	+	+
	10^-1	+	+	+	+
	10^-2	+	+	-	+
	10^-3	-	+	-	+
	10^-4	-	+	-	-
		SpeK		Internal control
7	10⁰	+	+	+	+
	10^-1	+	+	+	+
	10^-2	-	+	+	+
	10^-3	-	+	+	+
	10^-4	-	-	+	+

* For each 10-fold DNA dilution, the lowest level of detection of the duplex real-time PCR is shown in addition to the three 10-fold dilutions immediately prior to the lowest limit. Each dilution series was analysed in triplicate to determine relative sensitivity.

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ISSN: 1476-0711

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