Table 1 Duplex real-time PCR primers and probes used in the detection of streptococcal SAgs
Groups | Name | Sequence (5′-3′) |
|---|---|---|
1 | SpeA | Forward: CAAGAGGTATTTGCTCAACAAG |
Reverse: CACATTCTCGTGAGTAACAGG | ||
Probe: FAM-CCCGATCCAAGCCAACTTCACAGA-BHQ1 | ||
SpeL | Forward: GTCAGCACCTTCCTCTTTC | |
Reverse: ATCTCCCGTTACCTTCCAG | ||
Probe: HEX-CGCCTGAGCCGTGAAA-BHQ1 | ||
2 | SpeB | Forward: CGGACGTAACTTCTACCATG |
Reverse: TTTGATGCCTACAACAGCAC | ||
Probe: FAM-AACCGTTGAAGCCGCC-BHQ1 | ||
Ssa | Forward: CGGGAACTTACCTTCAATTTG | |
Reverse: CTATAACTGCTATTATTCGGAAGG | ||
Probe: HEX-TGCTCAGTAACACCTCC-BHQ1 | ||
3 | SpeC | Forward: CGTAACTTTCCAGGAAATTGAC |
Reverse: GCTCATGTTTCCCATCTTTTG | ||
Probe: FAM-CGATTCTGCCGCTTACA-BHQ1 | ||
SpeH | Forward: AGTTCCTGTAAACGTGTGG | |
Reverse: CCTGAGCGGTTACTTTCG | ||
Probe: HEX-AACAACAGCCGCCTATG-BHQ1 | ||
4 | SpeG | Forward: AACAGTTTACTTTACAGGAATTTG |
Reverse: TGTTTACTATCTTTAGTAGCAAGG | ||
Probe: FAM-AAAGGCTCCCCGATGT-BHQ1 | ||
SpeF | Forward: CATTGATACCACGACAGCTCTTG | |
Reverse: TAGAAGCAAATCGTGATGGCTATC | ||
Probe: HEX-AGCCGCTCCAATCTACAACGCAGACGA-BHQ1 | ||
5 | SpeM | Forward: TTCGCTATTAACCAACACAGCATC |
Reverse: TGTTATTCCTTGTGTGTGTATCGC | ||
Probe: FAM-AAGACACTCTCAGTAG-NF-BHQ1 | ||
SpeI | Forward: ATATGATCCAACAGAAGTAAAAGG | |
Reverse: CAATCGAACTATTGCCATAAGG | ||
Probe: HEX-AATGAAGGTCCGCCA-BHQ1 | ||
6 | SpeJ | Forward: TCTACTGGTATGATTTCGTATGCG |
Reverse: TCATGGGTACGGAAGTGTAAAATC | ||
Probe: FAM-TTGTAGCTTAACGTC-NF-MGB | ||
SmeZ | Forward: GCCAATGATTTTAAAGMTGGAG | |
Reverse: TATATGCTGTGACTTTTCCTTTTG | ||
Probe: HEX-CTGTGTTCTCCGTCCCA-BHQ1 | ||
7 | SpeK | Forward: ACGTATCTGAAAAGACACTCTC |
Reverse: ATACCTTGACTTTGTTATTCCTTG | ||
Probe: FAM-AGAGCAAGCGATACACA-BHQ1 | ||
Internal control | Forward: GGTGTATAACGTGTCAGAGACC | |
Reverse: CTTCTCCAACATACTATGCAAC | ||
Probe: HEX-CCTGTCCACCTTCCTC-BHQ1 |