Figure 5
Ultrathin sections of C. albicans (isolate 77) control and treated with 1 μg/ml of WSP1267 [IC 50 ] for 48 h at 35°C. Untreated yeast (A) present a preserved ultrastructure (fibrillar structures - f, cytoplasm membrane - cm, and compact cell wall - cw). However, in treated yeasts (B-D), different alterations can be observed: disruption of cell wall and loss of cell wall integrity in the bud (B, black arrow), detachment of cytoplasmic membrane from the cell wall (B-C, arrow), presence of small vesicles in the periplasmic region (3B-D and inset in 3C) and large electron-dense vacuoles [v] (Figure 3D). Bars = 1 μm.