Figure 3

DIC microscopy (above) and fluorescence microscopy with DAPI (below) of C. albicans (isolate 77) untreated in different phases of cell cycle. Treatment with 1 μg/ml WSP1267 [IC₅₀] for 48 h at 35°C induced alterations in the cell cycle (white bars) in comparison with untreated yeasts (grey bars). The graphic revealed a significant increase in the number of yeasts with alterations in the cell cycle (decrease in phase I and increase in phases II and III). Cell cycle phases: (I) cells with one nucleus, (II) cells with bud and one nucleus in the mother cell, and (III) cells with bud and two nuclei (one in each cell). Relevant yeast from each phase is indicated by white arrows. Bars = 5 μm; * p < 0.05; ** p < 0.01.