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Table 1 Antifungal interactions (FICI) between complex II and III inhibitors tested against filamentous fungi in microtiter plates1.

From: Antifungal activity of redox-active benzaldehydes that target cellular antioxidation

Combinations A MIC alone MIC combined FICI MIC alone MIC combined FICI MIC alone MIC combined FICI
Strains A. terreus UAB698    A. terreus UAB680    A. terreus UAB673   
Kre-Me
Carboxin
> 1.62
> 1283
0.2 - 0.4
8 - 16
0.19 S > 1.6
> 128
0.2 - 0.4
4 - 8
0.16 S > 1.6
> 128
0.2 - 0.4
8 - 16
0.19 S
Pyraclostrobin
Carboxin
> 1.6
> 128
0.0 - 0.05
4 - 8
0.05 S > 1.6
> 128
0.0 - 0.05
4 - 8
0.05 S > 1.6
> 128
0.0 - 0.05
4 - 8
0.05 S
AntimycinA
Carboxin
> 1.6
> 128
0.4 - 0.8
8 - 16
0.31 S > 1.6
> 128
0.4 - 0.8
16 - 32
0.38 S > 1.6
> 128
0.4 - 0.8
16 - 32
0.38 S
Strains A. flavus NRRL3357    A. fumigatus AF293    P. expansum NRRL974   
Kre-Me
Carboxin
0.4 - 0.8
> 128
0.0 - 0.05
0.5 - 1
0.07 S > 1.6
> 128
0.0 - 0.05
2 - 4
0.03 S 0.4 - 0.8
32 - 64
0.0 - 0.05
0.5 - 1
0.08 S
Pyraclostrobin
Carboxin
0.4 - 0.8
> 128
0.0 - 0.05
0.125 - 0.25
0.06 S 0.8 - 1.6
32 - 64
0.0 - 0.05
1 - 2
0.06 S 0.4 - 0.8
2 - 4
0.0 - 0.05
0.25 - 0.5
0.19 S
AntimycinA
Carboxin
0.4 - 0.8
> 128
0.2 - 0.4
4 - 8
0.53 A 0.8 - 1.6
> 128
0.2 - 0.4
4 - 8
0.28 S 0.4 - 0.8
> 128
0.05 - 0.1
2 - 4
0.14 S
Combinations B MIC alone MIC Combined FICI MIC alone MIC combined FICI MIC alone MIC combined FICI
Strains A. terreus UAB698    A. terreus UAB680    A. terreus UAB673   
Kre-Me
TTFA
> 1.62
> 1283
0.2 - 0.4
4 - 8
0.16 S > 1.6
> 128
0.2 - 0.4
16 - 32
0.25 S > 1.6
> 128
0.2 - 0.4
16 - 32
0.25 S
Pyraclostrobin
TTFA
> 1.6
> 128
0.1 - 0.2
4 - 8
0.09 S > 1.6
> 128
0.2 - 0.4
4 - 8
0.16 S > 1.6
> 128
0.2 - 0.4
4 - 8
0.16 S
AntimycinA
TTFA
> 1.6
> 128
0.4 - 0.8
32 - 64
0.50 S > 1.6
> 128
0.4 - 0.8
32 - 64
0.50 S > 1.6
> 128
0.8 - 1.6
32 - 64
0.75 A
Strains
Compounds
A. flavus NRRL3357    A. fumigatus AF293    P. expansum NRRL974   
Kre-Me
TTFA
> 1.6
> 128
0.4 - 0.8
16 - 32
0.38 S > 1.6
> 128
0.2 - 0.4
8 - 16
0.19 S > 1.6
16 - 32
0.4 - 0.8
4 - 8
0.50 S
Pyraclostrobin
TTFA
> 1.6
> 128
0.4 - 0.8
8 - 16
0.31 S > 1.6
> 128
0.1 - 0.2
4 - 8
0.09 S > 1.6
4 - 8
0.05 - 0.1
1 - 2
0.28 S
AntimycinA
TTFA
> 1.6
> 128
> 1.6
> 128
1.00 N > 1.6
> 128
0.8 - 1.6
32 - 64
0.75 A > 1.6
> 128
0.00 - 0.05
8 - 16
0.08 S
  1. 1 Compound interactions were determined as Fractional Inhibitory Concentration Indices (FICI), described by Isenberg ([47]; See also Methods). For calculation purposes, the higher concentration in each column was used. A, additive; N, neutral; S, synergistic. Complex II inhibitors (μg/ml)- Carboxin, Thenoyltrifluoroacetone (TTFA); Complex III inhibitors (mM)- Kresoxim methyl (Kre-Me), Pyraclostrobin, Antimycin A. Drug combinations for calculating FIC indices were: (Combinations A) Kre-Me + carboxin, pyraclostrobin + carboxin, and antimycin A + carboxin, and (Combinations B) Kre-Me + TTFA, pyraclostrobin + TTFA, and antimycin A + TTFA.
  2. 2 Since antifungal test was performed up to 1.6 mM of strobilurins or antimycin A (See Methods), 3.2 mM (doubling of 1.6 mM) was used for calculation purposes.
  3. 3 Since antifungal test was performed up to 128 μg/ml of carboxin or TTFA (See Methods), 256 μg/ml (doubling of 128 μg/ml) was used for calculation purposes.