Biofilm formation and adherence characteristics of an Elizabethkingia meningoseptica isolate from Oreochromis mossambicus

Table 1 Biofilm formation by Elizabethkingia meningoseptica isolates CH2B and NCTC 10016^T following incubation at room temperature (~21°C) or 37°C, under static or dynamic conditions in nutrient-poor (EAOB) media or nutrient-rich (TSB), respectively

Parameters	Biofilm formation (OD_{595 nm}± SD)^a
	*Elizabethkingia meningoseptica*				*Vibrio mimicus*
	CH2B	BF ^b	NCTC 10016 ^T	BF ^b	VIB1	BF ^b
21°C EAOB dynamic	0.23 ± 0.02	M	0.07 ± 0.01	N	1.34 ± 0.28	S
21°C EAOB static	0.31 ± 0.13	M	0.16 ± 0.01	W	1.01 ± 0.18	S
21°C TSB dynamic	0.44 ± 0.14	S	0.25 ± 0.01	M	0.11 ± 0.00	W
21°C TSB static	0.56 ± 0.06	S	0.26 ± 0.02	M	0.14 ± 0.07	W
37°C EAOB dynamic	0.38 ± 0.19	M	0.11 ± 0.07	W	0.30 ± 0.09	W
37°C EAOB static	0.46 ± 0.35	M	0.27 ± 0.06	M	0.37 ± 0.15	W
37°C TSB dynamic	0.84 ± 0.17	S	0.36 ± 0.02	S	0.34 ± 0.39	M
37°C TSB static	0.90 ± 0.07	S	0.42 ± 0.05	S	0.27 ± 0.34	M

^a Biofilm formation assay data is the mean of three independent experiments carried out in triplicate ± standard deviation following growth in minimal (EAOB) or rich (TSB) media at 21 or 37°C under dynamic or static conditions, respectively.
^b Biofilm formation (BF) was classified as non-adherent (N), weakly (W)-, moderately (M)- or strongly (S)-adherent using previously described criteria [12].

ISSN: 1476-0711