|
Isolates
|
Broth dilution
μg/ml
|
Agar dilution
μg/ml
|
E-test
μg/ml
|
VITEK 2
μg/ml
|
MET
μg/ml
|
GRD strip
μg/ml
|
MHA5T
|
|---|
| |
VAN
|
TEIC
|
VAN
|
TEIC
|
VAN
|
TEIC
|
VAN
|
TEIC
|
VAN
|
TEIC
|
VAN
24 h/48 h
|
TEIC
24 h/48 h
|
TEIC
|
|
SaU
|
1
|
1
|
1
|
1
|
1
|
1
|
1
|
≤ 05
|
4
|
8
|
1/1
|
2/2
|
-
|
|
SaB1
|
1 (1)*
|
1
|
1
|
1
|
1 (1.5)*
|
1 (1)*
|
1
|
≤ 05
|
4
|
8
|
1/1
|
2/2
|
-
|
|
SaB2
|
2 (2)*
|
4
|
2
|
4
|
2 (2)*
|
2 (1.5)*
|
2
|
4
|
12
|
12
|
4/4
|
12/16
|
+
|
- MIC: Minimum inhibitory concentration, VAN: vancomycin, TEIC: teicoplanin, MET: Macromethod E-test, 200 μl of inoculum at 2 McFarland standard suspensions onto BHI Agar, positive result: VAN MIC of ≥8 and TEIC MIC of ≥8 μg/ml or TEIC MIC of ≥12 μg/ml; GRD strip, Etest Glycopeptide-resistance (AB bioMérieux) detection strip were used as described by the manufacture:GRD positive (GISA o h-GISA): VAN o TEIC ≥ 8 μg/ml; MHA5T screening, Mueller Hinton Agar with 5 μg/ml of TEIC, 10 μl of inoculum at 0.5 McFarland-standard direct-colony suspensions. Any growth was considered positive. All screening methods were read at 24 and 48 h and incubated at 35°C. (MIC)*: MIC results from the Instituto Nacional de Enfermedades Infecciosas Dr. Carlos G. Malbran. The strains h-VISA-Mu3 and VISA-Mu50 and the vancomycin-susceptible S. aureus strain ATCC 29213 were tested for all methods in parallel, as positive and negative controls, respectively.
