Table 2 Bacterial cultures used in the study to determine the specificity of developed qPCR assay S664

Burkholderia pseudomallei (n = 17)

Standard strains

NCTC 13392, NCTC 6700, NCTC 4845, NCTC 10274

4 (purified DNA)

Positive (4)

Clinical isolates

Clinical isolates

9 (purified DNA)

Positive (9)

Soil isolates

Soil isolates

4 (purified DNA)

Positive (4)

Burkholderia/Delftia/Ralstonia (n = 9)

Burkholderia thailandensis

Clinical isolate

1 (purified DNA)

Negative (1)

Burkholderia mallei

NCTC 10245

1 (purified DNA)

Negative (1)

Burkholderia cepacia

MTCC 1617, MTCC 438

2 (purified DNA)

Negative (2)

Burkholderia gladioli

MTCC 1888

1 (purified DNA)

Negative (1)

Delftia acidovorans

MTCC 104

1 (purified DNA)

Negative (1)

Ralstonia eutropha

MTCC 1285

1 (purified DNA)

Negative (1)

Ralstonia insidiosa

ATCC 49129

1 (purified DNA)

Negative (1)

Ralstonia pickettii

MTCC 648

1 (purified DNA)

Negative (1)

Biothreat agents/simulants (n = 9)

Bacillus anthracis

Clinical isolate

2 (purified DNA)

Negative (2)

Bacillus globigii

ATCC 9372

1 (purified DNA)

Negative (1)

Brucella abortus

NCTC 11363

1 (purified DNA)

Negative (1)

Brucella canis

NCTC 11365

1 (purified DNA)

Negative (1)

Brucella melitensis

NCTC 10094

1 (purified DNA)

Negative (1)

Coxiella burnetii

Nine mile I

1 (purified DNA)

Negative (1)

Francisella tularensis LVS

NCTC 10857

1 (purified DNA)

Negative (1)

Pantoea agglomerans

ATCC 33243

1 (purified DNA)

Negative (1)

Bacteria of clinical relevance (n = 15)

Brevundimonas diminuta

ATCC 11568

1 (purified DNA)

Negative (1)

Corynebacterium pseudotuberculosis

MTCC 3158

1 (purified DNA)

Negative (1)

Escherichia coli

ATCC 35218

1 (purified DNA)

Negative (1)

Klebsiella pneumoniae

ATCC 27736

1 (purified DNA)

Negative (1)

Ochrobactrum oryzae

MTCC 4195

1 (purified DNA)

Negative (1)

Pasteurella multocida

MTCC 1148

1 (purified DNA)

Negative (1)

Pasteurella pneumotropica

MTCC 656

1 (purified DNA)

Negative (1)

Proteus vulgaris

ATCC 6380P

1 (purified DNA)

Negative (1)

Pseudomonas aeruginosa

ATCC 15442

1 (purified DNA)

Negative (1)

Pseudomonas citronellolis

MTCC 1191

1 (purified DNA)

Negative (1)

Pseudomonas putida

MTCC 102

1 (purified DNA)

Negative (1)

Salmonella typhi

Lab culture

1 (purified DNA)

Negative (1)

Shigella dysenteriae

Lab culture

1 (purified DNA)

Negative (1)

Staphylococcus aureus

ATCC 11632

1 (purified DNA)

Negative (1)

Yersinia enterocolitica

ATCC 55075

1 (purified DNA)

Negative (1)

Plant pathogen/symbionts (n = 5)

Pseudomonas syringae

MTCC 1604

1 (purified DNA)

Negative (1)

Rhizobium meliloti

MTCC 3402

1 (purified DNA)

Negative (1)

Rhizobium radiobacter

MTCC 6702

1 (purified DNA)

Negative (1)

Rhizobium rhizogenes

MTCC 2364

1 (purified DNA)

Negative (1)

Rhizobium trifoli

MTCC 905

1 (purified DNA)

Negative (1)

Other bacteria (n = 10)

Bacillus mycoides

MTCC 7538

1 (purified DNA)

Negative (1)

Bacillus thuringiensis

NCIM 5112,

MTCC 868, MTCC 869

3 (purified DNA)

Negative (3)

Corynebacterium ammoniagenes

MTCC 1816

1 (purified DNA)

Negative (1)

Corynebacterium callunae

MTCC 700

1 (purified DNA)

Negative (1)

Corynebacterium glutamicum

MTCC 26

1 (purified DNA)

Negative (1)

Microbulbifer elongatus

MTCC 2426

1 (purified DNA)

Negative (1)

Pseudomonas fragi

MTCC 510

1 (purified DNA)

Negative (1)

Vibrio fischeri

MTCC 1738

1 (purified DNA)

Negative (1)