Table 2 Limit of detection using Probit regression analysis for the combined CRISPR/Cas-LAMP assay. (Values indicated in bold are the LOD was 74 N. meningitidis copies
From: An isothermal CRISPR- based lateral flow assay for detection of Neisseria meningitidis
Probability | 95% Confidence limit | ||
|---|---|---|---|
Estimate | Lower Bound | Upper Bound | |
0.010 | -102.578 | -211.139 | -61.796 |
0.020 | -90.449 | -188.779 | -53.383 |
0.030 | -82.754 | -174.605 | -48.033 |
0.040 | -76.965 | -163.950 | -44.000 |
0.050 | -72.257 | -155.289 | -40.714 |
0.060 | -68.249 | -147.922 | -37.912 |
0.070 | -64.735 | -141.466 | -35.451 |
0.080 | -61.588 | -135.690 | -33.244 |
0.090 | -58.727 | -130.440 | -31.233 |
0.100 | -56.093 | -125.610 | -29.380 |
0.150 | -45.187 | -105.652 | -21.667 |
0.200 | -36.519 | -89.848 | -15.478 |
0.250 | -29.083 | -76.350 | -10.109 |
0.300 | -22.405 | -64.296 | -5.220 |
0.350 | -16.217 | -53.206 | − 0.610 |
0.400 | -10.345 | -42.784 | 3.867 |
0.450 | -4.664 | -32.835 | 8.333 |
0.500 | 0.927 | -23.234 | 12.917 |
0.550 | 6.518 | -13.910 | 17.779 |
0.600 | 12.199 | -4.858 | 23.142 |
0.650 | 18.070 | 3.855 | 29.327 |
0.700 | 24.258 | 12.112 | 36.771 |
0.750 | 30.936 | 19.863 | 45.963 |
0.800 | 38.372 | 27.298 | 57.395 |
0.850 | 47.040 | 34.918 | 71.767 |
0.900 | 57.946 | 43.633 | 90.723 |
0.910 | 60.580 | 45.651 | 95.388 |
0.920 | 63.442 | 47.817 | 100.483 |
0.930 | 66.588 | 50.173 | 106.111 |
0.940 | 70.102 | 52.776 | 112.424 |
0.950 | 74.110 | 55.715 | 119.654 |
0.960 | 78.819 | 59.136 | 128.181 |
0.970 | 84.608 | 63.304 | 138.701 |
0.980 | 92.303 | 68.795 | 152.734 |
0.990 | 104.431 | 77.369 | 174.933 |