Fig. 3
From: An isothermal CRISPR- based lateral flow assay for detection of Neisseria meningitidis
(A) N. meningitidis-specific LAMP assay amplicons visualized in an agarose gel (HO: negative control; human DNA: negative control; lanes 3–5 show the tested N. meningitidis concentrations 100: zero copies/ μL; 101: ten copies/ μL; 102: 100 copies/ μL). (B) Fluorescent signals detected by real-time PCR after CRISPR/Cas cleavage (red colour indicates 10 and 100 copies); green colour indicates human DNA and water as negative controls and 100: zero copies/μL). (C) Readout from the lateral flow assay. The test cassette shows two lines for negative samples (un cleaved) and one line for positive samples (cleaved)