Table 4 Quadruplex ddPCR results of follow-up MDR-TB patient samples
Sample No. | Sample type | Tx Hx | AFB smear | Molecular resistance (sequencing) | Phenotypic resistance | Culture | ddPCR (copies/reaction) | Xpert | Probe | |
|---|---|---|---|---|---|---|---|---|---|---|
MTB | RIF | |||||||||
G-2a | Sputum | 17 months of Tx | - | INH, RIF | INH, RIF, EMB, PZA | Negative | - | Negative | Negative | - |
G-5.2 | Sputum | 2 days of HERZ | 2+ | NT | NT | NT | IS6110 (2.33) | Negative | Negative | - |
G-6.2 | Bronchial lavage | 3 months of HERZ | - | NT | NT | Negative | IS6110 (268.66) | NT | NT | NT |
inhA (15.46) | ||||||||||
S-1 | Sputum | 3 weeks of HERZ | - | NT | NT | Positive | IS6110 (162.28) | Positive | Positive | probe E |
inhA (15.20) | ||||||||||
rpoB (13.03) | ||||||||||
S-3 | Sputum | 2 months of HERZ, 3 weeks of Z + H + Q | 1+ | INH, RIF | INH, RIF, EMB | Positive | IS6110 (195.64) | Positive | Positive | probe E |
inhA (20.81) | ||||||||||
rpoB (7.66) | ||||||||||
embB (14.54) | ||||||||||
rpsL (3.11) | ||||||||||
S-4b | Sputum | 1year of Tx (on and off) | 2+ | NT | NT | Positive | IS6110 (124.50) | Positive | Positive | probe E |
inhA (4.36) | ||||||||||
rpoB (10.89) | ||||||||||
S-7 | Sputum | 4 months of HERZ | - | NT | NT | Negative | IS6110 (9.93) | Positive | Positive | probe A |
inhA (3.31) | ||||||||||
rpoB (1.14) | ||||||||||
S-9 a | Sputum | 3 months of HERZ | - | RIF | INH, RIF, EMB, PZA, FQ, SM | NT | IS6110 (6.23) | Positive | Positive | probe E |
inhA (2.08) | ||||||||||
S-13 a | Sputum | 2 months of HERZ | - | INH, RIF | INH, RIF, EMB, SM | Positive | - | Positive | Positive | probe E |
S-14 | Sputum | 1 months of HERZ | - | NT | NT | Negative | IS6110 (11.38) | Positive | Positive | probe B |