Table 3 Quadruplex ddPCR results of initial MDR-TB patient samples
Sample No. | Sample type | AFB smear | Molecular resistance (sequencing) | Phenotypic resistance | Culture | ddPCR (copies/reaction) | Xpert | Probe | |
|---|---|---|---|---|---|---|---|---|---|
MTB | RIF | ||||||||
G-1 | Sputum | - | INH, RIF | INH, RIF, PZA | Positive | IS6110 (32.41) | Negative | Negative | - |
inhA (36.02) | |||||||||
G-3 | Sputum | - | INH, RIF | INH, RIF, PZA | Positive | IS6110 (45.29) | Positive | Positive | probe E |
inhA (9.93) | |||||||||
rpoB (2.21) | |||||||||
G-4 | Sputum | - | INH | INH | Positive | IS6110 (1493.43) | Positive | Negative | - |
katG (66.58) | |||||||||
inhA (75.95) | |||||||||
G-5.1 | Bronchial lavage | 2+ | INH | Pan-susceptible | Positive | IS6110 (63.65) | Positive | Negative | - |
katG (7.67) | |||||||||
G-6.1 | Sputum | - | INH | Pan-susceptible | Positive | IS6110 (946.90) | NT | NT | NT |
inhA (44.35) | |||||||||
G-7 | Sputum | - | INH | Pan-susceptible | Positive | IS6110 (160733.52) | NT | NT | NT |
inhA (6236.44) | |||||||||
S-2 | Sputum | 3+ | INH, RIF | INH, RIF, EMB | Positive | IS6110 (11.00) | Positive | Positive | probe E |
inhA (3.30) | |||||||||
rpoB (2.20) | |||||||||
S-8a | Sputum | 2+ | - | - | Positive | IS6110 (1302.16) | Positive | Positive | probe B |
katG (67.68) | |||||||||