Table 2 Resistance phenotype and ddPCR results of eight live MTB strains
Sample No. | Phenotypic resistance (Lowenstein-Jensen medium) | Molecular resistance (ddPCR) | Discrepancies between phenotype and genotype | Results of confirmation using Sanger sequencing |
|---|---|---|---|---|
T-1 | INH, RIF | katG, rpoB | ||
T-2 | INH, RIF, EMB, SM | katG, rpoB | EMB; SM | embB (M306V, M306I) and rpsL (K43R): ND |
T-3 | INH, RIF, PZA | rpoB, embB | INH; embB | inhA (C(-15)T) and katG (S315T): ND embB (M306I): D |
T-4 | INH, RIF | inhA, rpoB | ||
T-5 | INH, RIF, SM | katG, rpoB, gyrA, rpsL | gyrA | gyrA (D94G): D |
T-6 | INH, RIF, EMB | katG, rpoB | EMB | embB (M306V, M306I): ND |
T-7 | INH, RIF, EMB, SM | katG, rpoB, embB, gyrA, rpsL | gyrA | gyrA (D94G): D |
T-8 | INH, RIF, PZA | katG, rpoB |