Table 1 Primer sequences for evaluation of the expression levels of biofilm formation genes
Gene | Primer sequence | Tm (°C) | References |
|---|---|---|---|
luxS-F | 5ʹ-AGT GAT GCC GGA ACG CGG-3ʹ | 60 | [7] |
luxS-R | 5ʹ-CGG TGT ACC AAT CAG GCT C-3ʹ | 60 | [7] |
mrkA-F | 5ʹ-ACG TCT CTA ACT GCC AGG C-3ʹ | 60 | [7] |
mrkA-R | 5ʹ-TAG CCC TGT TGT TTG CTG GT-3ʹ | 60 | [15] |
pgaA-F | 5ʹ-GCA GAC GCT CTC CTA TGT C-3ʹ | 60 | [7] |
pgaA-R | 5ʹ-GCC GAG AGC AGG GGA ATC-3ʹ | 60 | [7] |
wbbM-F | 5ʹ-ATG CGG GTG AGA ACA AAC CA-3ʹ | 60 | [7] |
wbbM-R | 5ʹ-AGC CGC TAA CGA CAT CTG AC-3ʹ | 62 | [7] |
wzm-F | 5ʹ-TGC CAG TTC GGC CAC TAA C-3ʹ | 62 | [7] |
wzm-R | 5ʹ-GAC AAC AAT AAC CGG GAT GG-3ʹ | 62 | [7] |
23S rRNA-F | 5ʹ-ATC GTA CCC CAA ACC GAC AC-3ʹ | 62 | [7] |
23S rRNA-R | 5ʹ-TTC TCC CGA AGT TAC GGC AC-3ʹ | 62 | [7] |