Fig. 5

a CGview comparison of pEGY_KP9814_MCR1 with similar IncHI2 mcr-1.1-bearing plasmids retrieved from NCBI database. Circles from outside to inside correspond to the coding sequence regions of pEGY_KP9814_MCR1 (dark blue) with its size indicated in the middle of the circles, pAN65-1 (pink), pKP14052-MCR-1 (turquoise), pKP121-1-mcr (orange), pEGY1-MCR-1 (purple), and pEGYMCR_IncHI2 (spring green). The labels in the outermost circle represent the annotation of genes related to antibiotic resistance (red), tellurium resistance (green), transposases (blue), and plasmid replication, transfer, and segregation (black). Genomic regions covered by BLASTn are represented by a solid color whereas white gaps indicate regions not covered by BLASTn; b schematic illustration of the genetic environment surrounding mcr-1.1 gene in pEGY_KP9814_MCR1 compared to previously reported plasmids recovered from K. pneumoniae and E. coli. The colored shading between genetic loci indicates homology with a percentage identity demonstrated within the shaded region. Red, blue, yellow, pink, light blue, green, and grey arrows represent open reading frames corresponding to mcr-1.1, ISApl1, pap2, PP2C-encoding gene, tyrosine-type recombinase/integrase, terY, and different hypothetical proteins, respectively. The figure was generated using Clinker software