Fig. 4

Schematic representation of the insertional inactivation disrupting mgrB locus along with an illustration of truncated mgrB gene. a Intact wild-type mgrB locus of K. pneumoniae HS11286 showing mgrB gene (blue arrow) and promoter region (PR) (light blue rectangle); b mgrB gene inactivated by ISKpn14 at nucleotide position + 81, c at nucleotide position + 35, d at nucleotide position + 118, and e at nucleotide position + 119; f mgrB gene disrupted by ISKpn26 at nucleotide + 74; g mgrB promoter region interrupted by ISKpn14 at nucleotide position − 37 when referring to the first nucleotide upstream mgrB start codon as − 1. Target sites duplication of 4 and 9 bp are underlined, while the black triangles represent the left and right inverted repeats of the insertion sequences. h Truncated mgrB showing the deletion of the terminal 13 nucleotides yielding a MgrB of 43 amino acids instead of 47, F44_W47del indicates the deletion of amino acids from position 44 (phenylalanine) to position 47 (tryptophan); i truncated mgrB of 133 bp sequence producing a MgrB of 44 amino acids, I45_W47del indicates the deletion of amino acids from position 45 (isoleucine) to position 47 (tryptophan). The diagram is not made to scale