Fig. 3

Colistin MIC and sequence analysis of colistin resistance genes/proteins in the tested K. pneumoniae isolates compared to the wild-type sequence of K. pneumoniae subsp. pneumoniae HS11286 (GenBank accession number: NC_016845.1). Mutations, deleterious amino acid substitutions, insertional inactivation, or deletion potentially associated with colistin resistance are shown in red bold format. Deleterious and neutral amino acid substitutions were evaluated by PROVEAN tool. Green bold mutation in mgrB upstream region at position − 10, when referring to the first nucleotide upstream mgrB start codon as − 1, is suspected to be associated with colistin resistance. Others refer to PhoP, ArnC, ArnD, ArnE, RamA, kdgR, yobH, yebO, yobF, and cspC with ∆ indicating partial deletion of yobH and complete deletion of yebO, yobF, and cspC. On the left, isolates are clustering based on their sequence types that are indicated above each branch of the phylogenetic tree. Isolate shown in bold format (K2) harbored plasmid-mediated mcr-1.1 gene. WT wild-type